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How Dual-Labeled Probes are Used in Curing Diseases

Summary: Dual-labeled probes offer insight into the fight against life-threatening diseases.


Dual-labeled oligonucleotide probes are one of the most common types of probes used in DNA synthesis. They have many medical benefits, as they are highly sensitive and allow for sequence specificity due to the groundbreaking design.


Although dual-labeled probes are used for many different reasons, including gene expression analysis and gene copy determination, one of the most important advances that geneticists have made in the medical field involve the use of probes for pathogen detection, and thus, the early discovery and treatment of many types of cancer.


Predictive medicine relies on the speed and accuracy of dual-labeled probes to locate tumors in early stages and keep track of their growth.


How do they work? Dual-labeled probes aid in the detection and curing of diseases through a process known as fluorescence resonance energy transfer (FRET).


A single-stranded oligonucleotide is labeled with two different colored dyes: a reporter dye and a quencher dye. The quencher is covalently attached to the 5? to 3? ends of an oligo. Typically, this works best for oligo sequences between twenty and thirty bases.


The probe emits a low level of fluorescence, and through hydrolysis, the reporter is released from the probe and then binds to the target DNA template. This process is known as hybridization, as the probe and the target bind together.


The emission of light that occurs during FRET can be viewed and tracked in real time with a PCR thermal cycler.


Bio: The Midland Certified Reagent Company sells modified oligonucleotides for DNA and RNA synthesis, and various research purposes.

Detection and Localization of Cancer Using Fluorescent Probes

Summary: Learn more about how beacon probes help scientists get closer to a cure for cancer.


A commonly used cytogenetic technique used to detect and localize tumor cells is known as fluorescence in situ hybridization (FISH). This involves a type of probe known as a molecular beacon probe, and is able to hone in on the location of tumor cells.


This method is non-radioactive and involves labeling molecular markers in order to more clearly detect hybridization. Medical techs seeking cancerous tumor cells look for specific complementary sequences of nucleic acids.


A typical synthetic beacon probe is long and thin, shaped like a hairpin. It is usually no more than 25 oligos long. Fifteen of the 25 nucleotides in a probe complement the desired target DNA or RNA, and the termini of the probe complement each other.


Biomedical researchers in the 1980s made the groundbreaking discovery that fluorescence microscopy can be used to detect a fluorescent probe binding to a specific chromosome. This is done by labeling the


When probes have a fluorescent label, the light emitted during the process of fluorescence resonance energy transfer (FRET) is visible in real time, ensuring that medical techs can very quickly locate tumor cells and track their metastasis.


While FISH is also occasionally used to identify species, its application in predictive and preventative medicine is very significant. Unusual temporal and spatial patterns of gene expression signify that cells and tissues are being invaded by a cancerous tumor, and FISH makes it easier for medical techs to find the tumor before it can metastasize.


Bio: The Midland Certified Reagent Company is a leader in the manufacture of polynucleotides for research purposes.










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