+(800) 247-8766

Cart

 

 

 

 

Welcome

Midland is a leader in the synthesis of DNA and RNA oligonucleotides for over 35 years. We pioneered the use of MALDI-TOF mass spectrometry.

 

Our Products

We continue to offer a wide range of polynucleotides, including duplexed polynucleotides and template/primer combinations. 

 

News

The Midland catalogue contains carries several varieties of DNA polynucleotides , DNA template/primers, and Homo Oligomers. 

 

 

Midland oligos are often used for gene synthesis in the following way. Two sets of 40-mers are synthesized, each set comprising one of the two strands of the desired gene. Each oligo overlaps a 20-base region of two oligos in the complementary set. The oligos are all combined in approximately equal amounts and several cycles of amplification by PCR are performed. Finally two oligos corresponding to the 5-ends of each of the strands are added and several more cycles of PCR are run. The products are purified by electrophoresis and the product of the desired size is collected...

If you wish to order Oligos

 

Copyright Midland Certified Reagent Company 2015

HOME   -   ABOUT US   -   OUR PRODUCTS   -   TECHNICAL INFORMATION   -   NEWS   -   CONTACT